Discovery and Genetic Mapping of PM1, a Powdery Mildew Resistance Gene in Cannabis sativa L.

Powdery mildew is among the most common diseases of both hemp- and marijuana-type cultivated Cannabis sativa . Despite its prevalence, no documented studies have characterized sources natural genetic resistance in this pathosystem. Here we provide evidence for first ( R ) gene C. , represented by a single dominant locus that confers complete to an isolate powdery pathogen Golovinomyces ambrosiae found Pacific Northwest United States. Linkage mapping with nearly 10,000 nucleotide polymorphism (SNP) markers revealed (designated PM1 located on distal end long arm one largest chromosomes genome. According reference whole genome sequences Sanger sequencing, marker was tentatively placed cluster genes nucleotide-binding site (NBS) leucine-rich repeat (LRR) protein type. 's behavior, qualitative penetrance, co-segregating qPCR track inheritance were confirmed two separate backgrounds totaling 185 recombinant F1 plants. The goal study foundation discovery characterization additional pathogens infect